EZ-Link(R) NHS-Chromogenic

Use of In Vivo Biotinylation for Chromatin Immunoprecipitation

for interpreting the data obtained from ChIP samples analyzed by qPCR, and methods for testing the efficiency of biotinylation using a streptavidin gel?shift are also presented. In addition, a complementary method, based on isothermal multiple strand displacement

Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation

Figure 1.B0.1 Establishment of a biotinylation system in J1 ESCs. A stable ESC line expressing the bacterial BirA enzyme was first established by transfection with a BirA‐expressing plasmid bearing the neomycin resistance ( neo r ) gene and G418

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis