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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
al 1992, Vaysse and Goldman, 1990). The indigo product of X-gal absorbs in the wavelengths emitted by the standard fluorescent-conjugated antibodies and is so dark that it can obscure the products from either horseradish peroxidase or alkaline
ALKALINE PHOSPHATASE REMOVAL OF PO4 FROM DNA FRAGMENTS
of Alkaline Phosphatase (AP) can hydrolyze 50 pmol of 5'' terminal phosphorylated DNA fragments (3'' recessed, 5'' recessed or blunt-ended) when incubated at 37o C for 1 hour. This implies that 25 pmol of DNA are dephosphorylated (at both ends) in one hour
Western Blotting with Alkaline Phosphatase Conjugates
buffer (1% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH ALKALINE PHOSPHATASE CONJUGATED ANTIBODIES Dilute the AP
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