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Calcium Sensor Dye eFluor® 514 10 x 50 ug
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文献和实验E.Z.N.A.® Dried Blood DNA Spin Protocol
speed for 20 seconds. 7. Transfer the lysate from step 5 into the column, and centrifuge at 10,000 x g for 1 min to bind the DNA. Discard the flow-through liquid and the collection tube. 8. Place the HiBind® DNA Mini Column into a NEW 2 ml
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
and then at 280 nm. The DNA concentration is calculated as follows: DNA concentration = Absorbance260 × 50 × (Dilution Factor) :g/ml Although the binding capacity of HiBind® DNA Column is around 35 ug, the yield of the yeast plasmid depends
Use a 1L bag for CD3 T cell separation. Add 100 ml of air to the bag. 3) Add 5 x 108 CD3 T cells to the 1L bag in 50-60 ml Buffer 1. 4) Add 4.0 ml (corresponding to 1.6 x 109 Dynabeads®) of washed Dynabeads® CD3⁄CD
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