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Anti-Mouse CD4 PerCP-Cy5.5 100 ug
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文献和实验mm dish. This is about 100 ug protein for PC12 cells. After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer
Transgenic Mouse Core Facility
1.Restrict DNA and gel purify Cut 100ug DNA, removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of Tris saturated phenol with b
1. Cut 0.5 cm of tail and mark mouse ears or toes 2. Incubate at 55°C O/N in: 500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml) 12.5 l 20% SDS 3. Add 150 l 5 M NaCl
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