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Mouse HMGB1 Recombinant Protein Carrier-Free 500 ug
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文献和实验Th17 Polarization of Mouse CD4 Cells
Anti-mouse CD28, clone37.51[Details: LEAF™format, cat.#102112] Anti-mouse IL-4 [Details: LEAF™format, cat#504108] Anti-mouse IFN-γ [Details: LEAF™format, cat.#505812] Recombinant mouse IL-6(carrier-free)[Details: Biolengend, cat
) to remove free Ca++ . Use 100 g chelex resin per 500 ml FBS. Swell 100 g chelex resin in 400-500 ml distilled water, then titrate to pH 7.4 with HCl while stirring (pH will take a while to stabilize during titration). Filter through Whatman #1 paper. Scrape
Purification of recombinant sBRF M166L
under lac iQ control Kanomycin resistant) Inoculate 6 ml of a saturated overnight culture of the transformed bacteria into 2X 1 Liter of YT Amp (50 ug/ml) (and Kan (30 ug/ml) if using strain JM25) (adding 1-2 drops of antifoam A before autoclaving the YT
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