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Anti-Mouse CD25 APC 50 ug
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文献和实验and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Transgenic Mouse Core Facility
pellet in 100ul 10mM Tris, 0.1mM EDTA. Put through a millipore basket filter as described above. 4.Quantitate/Validate DNA Make a 1:50 dilution and read A260/A280 on spectrophotometer with the microcuvette. (Pure DNA A260/A280=1.8) Cut 1 ug insert
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