Mouse CXCL10 (IP-10) Recombina

一篇搞定 IP、co-IP 实验

了一线技术支持常被问到的问题，希望这篇纯干货能帮助大家顺利升级打怪，早日驾驭 IP、co-IP。 IP、co-IP、pull-down 简介 IP（Immunoprecipitation），免疫沉淀：是利用固定在磁珠或琼脂糖树脂等基质上的特异性抗体对抗原进行小型亲和纯化的一种方法 Co-IP（co-Immunoprecipitation），免疫共沉淀： co-IP 是一种常用的鉴定蛋白-蛋白相互作用的方法，其基本实验流程和IP类似，通过使用诱饵蛋白（IP 中的抗原，bait protein）特异

Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF- Moiety

(9 ). Because its systemic administration was shown to mediate the regression of some mouse tumors (10 ), TNF-α has attracted much attention as a potential antitumor reagent (11 ). However, the problem of its dose-dependent toxicity has been particularly apparent in human

Purification of recombinant sBRF M166L

Renaturation of BRF protein Thaw Ni-NTA purified samples. Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer. To the BRF sample, add 10% Brij58 detergent to a final concentration of 0.1% Brij, add DTT to 5 mM final concentration