MUC1 protein (His tag)

MUC1 Expression in Lung Cancer

and extensive O-glycosylation at serine and threonine residues, resulting in a composition of 50-80% carbohydrate by weight. To date, at least 9 mucin genes, encoding the protein backbone of these glycoproteins, have been identifi ed and at least partially

干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密

CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪

Phos-tag Affinity Electrophoresis for Protein Kinase Profiling

Protein kinase profiling can provide a basis for understanding the molecular origins of diseases and, potentially, for developing tools for therapeutic intervention. It is therefore very important to develop advanced