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- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Buffer RPE (55/250) N,KG
- 供应商:
北京智杰方远科技
- 保存条件:
详见说明
- 规格:
**
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文献和实验CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
buffer 2.5% Triton X-100 Protease analysis; renatures enzymes after electrophoresis 10x zymogram development buffer 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, 0.02% Brij-35 Protease analysis; activates enzymes after electrophoresis Premixed
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