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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
CloneJET™ PCR Cloning Kit
- 供应商：
北京智杰方远科技
- 保存条件：
详见说明
- 规格：
40 react
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文献和实验: If you have more than one PCR product, you may wish to gel-purify your fragment using the S.N.A.P.™ MiniPrep Kit. After purification,add Taq polymerase buffer, dATP, and 0.5 unit of Taq polymerase, and incubate 10-15 minutes at 72℃. Proceed directly to thecloning reaction
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
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