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- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
PageRuler Prest Protein Ladder, 10 x 250 µl
- 供应商:
北京智杰方远科技
- 保存条件:
详见说明
- 规格:
10 x 250 µl
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文献和实验Clonality - X Chromosome Inactivation Assay
microdissected cells in 20 µl proteinase K buffer. Incubate overnight at 37°C. B: DNA Purification Lyse the cells overnight at 42°C, in 50 µl phenol:chloroform:isoamyl alcohol. Boil 10 min at 95°C. Vortex 1 min. Centrifuge 20 min at 14,000 rpm at RT
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
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