Fetal Bovine Serum Tetracyclin

Serum is the blood component that can be obtained after coagulation and by removing cellular components.
Besides serum proteins it contains, e.g. growth factors, amino acids and hormones. This comprehensive mix makes
serum one of the most important supplements, supporting cells to grow and proliferate in in vitro cell culture.
Of special interest is the fetal bovine serum (FBS). It is especially rich in growth factors and is particularly low in
antibodies, which may influence the cell culture work.
Capricorn Scientific has established a test with an external test laboratory, using liquid chromatography tandem
mass spectroscopy to detect all kinds of tetracycline. This method allows an accurate detection of
Chlortetracycline and the Oxytetracycline in a range of 0.05 mg/l.
This product is used in cell culture applications using tet-inducible gene expression systems, and other applications in which the presence of tetracycline would prove disruptive.
 

Product	Origin	Volume	Cat. No.
Fetal Bovine Serum, Tetracycline Negative	Collected in South America	100 ml	CG-TET-12B
		500 ml	CG-TET-12A

Product Specifications

Appearance	Clear amber liquid
Storage and shelf life	Store at ≤-15°C.
	Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended.
	Once opened store at 4°C and use within 4-6 weeks.
Shipping conditions	Frozen (Dry ice)
Thawing	Overnight at +2°C to +8°C. Swirl gently to homogenize.

Additional Optional Treatments
Heat inactivation:
Heat inactivation will inactivate the complement system, antibodies and other active enzymes. It has to be done
in a carefully controlled process in order to avoid damaging the cell growth promoting properties of the serum
and reducing the formation of unwanted precipitates.
The process involves heating the serum in a shaking water bath at exactly +56°C for 30 minutes. The shaking will
help avoid the formation of protein and other forms of precipitates. After 30 minutes the serum is cooled back down  to room temperature as quickly as possible to avoid excessive exposure to heat which can damage e.g. growth factors and vitamins.

Gamma irradiation:
Capricorn Scientific has established process parameters and controls for maximum inactivation of contaminants by an innovative gamma irradiation process in small sized boxes at 25 – 35kGy. When FBS bottles are arranged in a pallet during gamma irradiation, high irradiation doses (58 kGy) are necessary to irradiate the centrally located
bottles with the required does (>30 kGy). This and the associated higher temperatures may affect the serum
quality.
If the irradiation is performed in single boxes, the maximum dose of irradiation does not exceed 38.7 kGy. Gentle
irradiation as used by Capricorn Scientific is less likely to affect final serum quality.