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文献和实验胚胎和成年斑马鱼眼情的组织学准备Histological Preparation of Embryonic and Adult Zebrafish Eyes
Reagents 0.01 M phosphate buffered saline (PBS) (pH 7.4) (Sigma) DPX mountant (Electron Microscopy Sciences, 13512) Embedding resin for zebrafish eyes
CONVENTIONAL TWO STEP FIXATION [See D.H. Hall, 1995]
M cacodylate on ice, 10 min. each 4. 0.5% OsO4, 0.5% KFe(CN)6 in 0.1M cacodylate, 90 min. on ice 5. 3 buffer washes, 10 min. each, 0.1 cacodylate; 3 buffer washes, 10 min. each (0.1M NaAcetate) 6. Stain in 1% UAc in 0.1M NaAcetate, pH 5.2, r.t., 60 min
to ensure collection of the sample. Resin binding A very popular method is to bind the DNA to silica particles by using commercially available binding resins, diatomaceous earth or glass fibers. This method was previously described in Methods
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