Fmoc-Thr(tBu)-CLEAR-Amide Resi

On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-Linked Oligosaccharide

This chapter describes a rapid and efficient approach for the solid-phase synthesis of N -linked glycopeptides that utilizes on-resin glycosylamine coupling to produce N -linked glycosylation sites. In this method, the full-length

Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries

(OBOC) libraries consist of structurally related compounds (e.g., peptides) covalently attached to a solid support, with each resin bead carrying a unique compound. OBOC libraries of high structural diversity can be rapidly synthesized and screened

Factor Xa Cleavage of MBP-Fusion protein

for each system. Samples must be removed at various time points and analyzed by PAGE-SDS to estimate the yield, purity and extent of factor Xa digestion. Factor Xa cleaves after the Arg residue in the cleavage site Ile-(Glu or Asp)-Gly-Arg. Ile and Thr are prefered