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- 文献和实验
- 技术资料
- 库存:
1
- 保存条件:
-20°c
- 供应商:
Merck
- 英文名:
MMP-14 Substrate I Fluorogenic
- 规格:
1MG
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文献和实验, and bronchoalveolar lavage, in which a multitude of hydrolases act in concert. This unit describes a protocol to map the global proteolytic substrate specificities of complex biological samples using a concise combinatorial library of internally quenched fluorogenic
A Method for Assaying Deubiquitinating Enzymes
as an excellent substrate for the sensitive and quantitative assay of various DUBs (13 ,14 ). Using this assay, we have also isolated a number of novel DUBs in chick skeletal muscle and yeast (13 , 15 -18 ). Here we describe the detailed protocol for assaying DUBs
Studies of the Ubiquitin Proteasome System
Materials Adherent cells expressing the substrate protein and E3 of interest (e.g., U2OS cells for p53 and MDM2), growing in 100‐mm dishes 50 mM MG132 (Calbiochem, Boston Biochem, or Biomol) in DMSO
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