QIAfilter Plasmid Maxi Kit (25

Fastfilter Plasmid Maxi Kit Spin Protocol

. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Maxi Column: 10. Transfer 16 ml of the cleared lysate to the HiBind® DNA maxi column assembled in the 50 ml collection tube. The Maxi column

QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒

      ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒

Fastfilter Plasmid Midi Kit Spin Protocol

plasmid purity. Prolonged incubation may lead to nicking of plasmid DNA. (Store Solution II tightly capped when not in use.) 5. Add 1.25 ml ice-cold Buffer N3, cover, and gently mix by inverting tube several times until a flocculent white precipitate