Type-it Microsatellite PCR Kit

FFPE Direct PCR Kit

down in microcentrifuge.    5) Set up program for a routine PCR reactions:       Initial Denaturation 94-95°C for 1-5 min       25-40 cycles 94-95°C for 30 sec                              45-70°C for 10-30 sec                              72

Plant Seed Direct PCR Kit

is covered by the Extraction Solution.    2) Incubate at 56°C for 1 hours.    3) Incubate at 95°C for 5 minutes.    4) Add 50 -100 μl PS3 Buffer and vortex to mix.    5) Store the extraction at 2-8°C. 3. PCR Protocol

Microsatellite Analysis in Human Disease

in the construction of human (and other model organisms) linkage maps. For instance, a recent human microsatellite linkage map consists of over 5264 (dA-dC)n loci with a heterozygosity >70% distributed along the genome at an average interval of 1.6 centiMorgans (CM