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Easy-Read Tubes, w/o prep
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文献和实验the pellet. Add 400 ul 75% EtOH, vortex briefly. Spin at 4°C, 4000 rpm for 10 min. Remove s/n with pipet and invert racked tubes in hood with door half shut. Approx. 10-15 minutes; do not overdry. Add 300 l DEPC-treated ddH2 O
4 Make a 10X stock Night before: Make 600 ml of 1X PBS 0.1X PBS 0.1X PBS, 8% (w/w) ultrapure sucrose 8% (w/w) ultrapure sucrose LB (w/o BME): add 280 ul BME before use LB + 0.5% NP-40 (w/o BME): add 280
27 Some sort of gradient fractionator with a fraction collector 16 30 ml corex tubes (cold) Solutions: A lot of these solutions are w/w; Tim says that to make these weigh out sucrose and then add buffer till weight is 100
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