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Buffer TCL, 2x (8,5/15),G
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文献和实验CCMB buffer to make chemical competent cell
but that amount was found to be 2X too much per tests on 1.23.07 --Meaganl 15:50, 25 January 2007 (EST) water to 1 liter autoclave or sterile filter Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5
In Situ Hybridization Using Digoxigenin Labeled Probes
with a scalpel blade. 6) Wash the slides 3 times 15 minutes in 2X SSC at 53°C. Proceed directly with the signal detection. It is important not to let the slide dry out during the signal detection procedure. Signal Detection: To detect, we use a 1:500
complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
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