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EpiTect Plus DNA Bisulfite Kit (48)
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文献和实验by adding 55 mg of hydroquinone to 50 ml of water. Add 520 ml freshly prepared 3M Sodium bisulfite (Sigma S-8890), prepared by adding 1.88 gm of sodium bisulfite per 5 ml of H2O, and adjusting pH to 5.0 with NaOH. Assure that reagents are mixed with DNA
Bisulfite modification kits and PCR-DNA Methylation, His
I was wondering if anyone could provide an opinion on the difference in PCR amplification effiency from DNA that has been Bisulphite modified using either the MethylEasy Kit or the EZ DNA methylation kit. We are trying to amplify a PCR product
Adapted from Frommer et.al.* 1.Dilute DNA (up to 2 mg) into 50 ml with distilled H2O. 2.Add 5.5 ml of 2M NaOH. 3.Incubate at 37℃ for 10 minutes (to create single stranded DNA ). 4.Add 30 ml of 10 mM hydroquinone (Sigma) to each tube, freshly
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