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- QIAGEN
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- 2025年11月17日
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QIAGEN Protease (30 AU)
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文献和实验Partial proteolysis with Staph. aureus V8 protease
Materials: Overlay/Rehydration buffer: 125 mM Tris-HCl, pH 6.8 1mM EDTA 0.1% SDS 1mM 2-b-mercaptoethanol 30% glycerol some Bromophenol blue For 1 ml, you will need 0.35 ml of 2X buffer 0.30 ml H20
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
(stored in 4℃) by vortexing repeatedly, leaving no clumps. Transfer the suspension into a round-bottom 13 ml polycarbonate tube. 4. Add 4 ml of buffer P2 (stored in Qiagen buffers box) and mix by covering with parafilm and inverting 4-6 times. Incubate
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