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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
WET FRED For 1 ml Gravity flow columns
- 库存:
999+
- 供应商:
IBA Lifesciences
- 规格:
1 set
Specifications
| Alternative Names: | Flow regulation device |
|---|---|
| Kit Content: | Column adapter, 2x tube/luer adapter female, 2x tube/luer adapter male, 20 ml syringe, 5 ml syringe, 6 cm tube, 100 cm tube, bottle neck adapter for column, last drop filter |
| Possible Application: | Sample application on gravity flow columns |
| Recommended Usage: | 1 ml gravity flow columns |
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文献和实验在哺乳动物和昆虫细胞表达系统中,目的蛋白经常分泌到细胞培养基。因此,在进行纯化前,我们的目的蛋白已存在于大体积培养液中。然而有时,用来纯化目的蛋白柱子体积却只有 1 ml。时间金贵,手动一次一次的将样品加入柱子中就太麻烦了,简直是要加到头秃有木有! 不知道大家为了简化操作,节省时间,都使用了怎样的方法来上样呢? 今天为在这里为大家分享一个锦囊工具:Wet Fred。这个小体积的工具非常灵活,可以根据我们的实验需要,使用在实验台、冰箱和冷室中。 优点 Wet Fred 工作
Southern Blotting(Fred Hutchinson Cancer Research Center)
) for 20' (small) to 30' (large). 4. Cut nylon membrane (MSI 0.45 micron #N04HY00010) and several pieces of blotting (e.g. Schleicher and Schuell GB002) paper to the same size as the gel. Wet the nylon with dH20, then soak in 5x SSC. 5. Assemble sandwich
Southern Blotting(Fred Hutchinson Cancer Research Cente
). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20' (small) to 30' (large). 4. Cut nylon membrane (MSI 0.45 micron #N04HY00010) and several pieces of blotting (e.g. Schleicher and Schuell GB002) paper to the same size as the gel. Wet the nylon











