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- 英文名：
NeXtal Stock Kit Buffer
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文献和实验Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Stock solutions for tissue culture
inhibit cell dispersal. Tryp/TD is 0.25% Bacto trypsin in TD. It is stored frozen. PBS is a phosphate-buffered physiological saline solution. It is useful if you want to avoid Tris buffer. Like Tris, it contains calcium and magnesium. There is a rarely
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