mRNA纯化试剂盒

mRNA Catcher™ PLUS Purification Kit
品牌：Ambion
mRNA的提取加纯化试剂盒

description

the mRNA catcher™Plus试剂盒enables高通量纯化的mRNA从组织和血细胞，总RNA，在96孔格式。以锁模模式的优势nucleic酸（LNA）of the mRNA的试剂盒catcher™Plus技术，提供改进的性能和协议是更快，同时保持mRNA和纯（see table）。offers mRNA catcher™Plus试剂盒：the

•结合能力的高鲁棒性能好（＞80 ng /孔）

结果-低•reproducible CVS从井和板-板（2 %）

•optimized protocols换小样品（100毫克的维大小. 4，组织，细胞，和血40µL）

•板兼容的设计与大多数PCR骑may be used

这样

从总RNA，mRNA，细胞，组织，和血samples is to an杂交固定化，单链20 mer Oligo（dT）中的价值。incorporation into the of the LNA寡核苷酸探针杂交效率和specificity increases the of the mRNA的结合。无论哪种好茶可以eluted mRNA或cDNA directly directly反相transcribed to the阱中。the method does not require mRNA catcher™加centrifugation相分离，沉淀，过滤，或柱。在加成，amenable method is to the全部换streamlined自动化高通量表达谱，为大尺度过程。适用于隔离mRNA在downstream applications is for such as使用RT-PCR和荧光实时定量PCR。

FastTrack™ MAG Micro mRNA Isolation Kit

(Ambion®)

Catalog Number
K1580-01

Size
12 preps
FastTrack™ MAG Maxi mRNA Isolation Kit

Catalog Number
K1580-02

Size
FastTrack™ MAG 96 mRNA Isolation Kit

Catalog Number
K1580-96

Size
2 x 96 preps

fasttrack™MAG微分离试剂盒mRNA

（ambion®）

目录编号

k1580 - 01

大小

为12

fasttrack™MAG马克西mRNA分离试剂盒

目录编号

k1580 02

大小

fasttrack™MAG 96 mRNA分离试剂盒

目录编号

k1580 96

大小

为2×96
mRNA Catcher PLUS Purification Kit

描述

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

• Robust performance—high binding capacity per well (>80 ng/well)
• Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
• Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
• Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.