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- 文献和实验
- 技术资料
- 英文名：
QX Pro Labeling Buffer (25 ml)
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文献和实验to about 100μl (for 2 mg vinculin)using a Centricon-30 (concentrate in a SS34 rotor at 6,500 rpm,4℃ for about 20 min.Then collect at 2,000 rpm for 3 min). 10.Dialyze overnight against buffer 6 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2
Labeling Muscle Actin with 5-iodoacetamidofluorescein
solution to actin and mix gently with a Pasteur pipte. 8.Let sit on ice for 2 hr. 9.Stop the reaction by adding DTT to 10 mM. 10.Dialyze against 1 liter buffer 3 overnight. Day 2 Materials 1.Buffer 1 as for day 1,4℃,2000 ml. 2.G-25-150 column,~30
LABELING MUSCLE ACTIN WITH IATR
pipet. 10.Let sit on ice for 2 hr. 11.Stop the reaction by adding DTT to 10 mM. 12.Dialyze against 1 liter buffer 1 overnight. Day 2 Materials 1.G-25-150 column,~30x1.5 cm. 2.Buffer 1 as for day 1,4℃,1000 ml. 3.50Ti tubes,volumetric conical tube
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