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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-70°c
- 供应商：
Merck
- 库存：
1
- 英文名：
ROSETTA-GAMI 2(DE3) COMPETENT CELLS
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
Transformation of plasmid DNA to competent E. coli cells Transformation is the process of getting the recombinant vector from a reaction mixture or vector solution into E. coli cells. To enable the cells to take up circular vector DNA
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