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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-70°c
- 库存：
1
- 英文名：
ROSETTA-GAMI B(DE3) COMPETENT CELLS
- 供应商：
Merck
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文献和实验Preparation of Competent Cells (from PMB)
the cells again (5 K, 5 min, 4 ℃). 4.Decant and resuspend in 1 ml PMB #B. Then add 9 ml more PMB #B. 5.Let sit on ice for 30 min, spin as above and resuspend in 2 ml PMB #B, add 0.2 ml glycerol and 30 µl DMSO. Use fresh or freeze in dry ice/EtOH. PMB
TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Transformation of Competent Cells
Transformation of Competent Cells A. Preparing competent cells. Inoculate 30 ml of SOB broth in a 250-ml flask with bacteria to be transformed from a single colony on a fresh plate.
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