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- 文献和实验
- 技术资料
- 英文名:
Gentamycin Sulfate Sterile-Filtered
- 库存:
1
- 供应商:
Merck
- 保存条件:
-20°c
- 规格:
20ML
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文献和实验Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
) in PBS, stock sterile filtered Lipopolysaccharide (LPS) from Salmonella typhimurium, 1000x stock [50 µg/ µL] B cell medium PBS Gentamycin 500x (50 mg/ mL) Puromycin 200x (300 µg/ mL) Hygromycin B 500x (50mg/ mL)
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
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