murine MAb against human tissu

Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium

or CELLstart™ CTS™ substrate Attachment Factor Stem Cell Tested 0.22 μm pore size sterile filters BD Falcon T175 TC-Flasks 37°C water bath Appropriate tissue culture plates and supplies 实验步骤   1. Preparing

Dual- and Triple-Color Fluorospot

labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml

Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma

Conventionally, monoclonal antibodies (MAbs) are generated by fusing B cells from an immunized animal with myeloma cells from the same species (1 ). Several murine MAb have already been employed for in vivo diagnosis and therapy, including