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- novagen
- 70979-3
- 2025年10月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°c
- 英文名：
BETAFLUOR B-GAL ASSAY KIT
- 供应商：
Merck
- 库存：
1
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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
5 × 106 cells/mL for each assay. Cells may be suspended in medium or buffer. 3. Add 50 μL DMSO (Component C) to one vial aqua-fluorescent reactive dye (Component B). 4. Add 42 μL DMSO (Component C) to one vial calcein violet
galactosidase assay for cells transfected with pSV- -Galactosidase Vector
absorbance at 420 nm. All components for b-gal assay are on top shelf of -20℃. All components for Luciferase assay are in Common Rack B. For info about this kit and map of pSV-b-gal vector, look in the cardboard box of kit manuals by Fan’s office.
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
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