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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
AssayPro
- 检测范围:
0.3 ng/ml
- 检测方法:
ELISA
- 应用:
血清、血浆、细胞培养上清液
- 样本:
50 μl
- 规格:
96 wells
猪凝血酶原PT酶联免疫试剂盒
Prothrombin is also known as Factor II. The conversion of Factor X to Xa changes prothrombin into its active form, thrombin, which then accelerates the formation of fibrin. The level of the plasma prothrombin in the circulating blood decreases during its passage through the pulmonary capillaries (1). The bleeding tendency in acute chloroform intoxication is due to deficiency in both plasma fibrinogen and plasma prothrombin (2). On the other hand, in severe Alzheimer's disease, prothrombin was localized within the wall and neuropil surrounding microvessels (3). It has been reported that plasma prothrombin level increases in sepsis patients (4), and in chronic gastrointestinal disorders (5).
Principal of the Assay
The AssayMax Swine Prothrombin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of swine prothrombin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine prothrombin in less than 4 hours. A monoclonal antibody specific for swine prothrombin has been pre-coated onto a 96-well microplate with removable strips. Prothrombin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for swine prothrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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文献和实验ResidueTest ® Estradiol ELISA kit 雌二醇 (E2) 酶联免疫试剂盒,(编号: HM2093 ),采用竞争酶联免疫分析法定量检测牛奶和奶牛血清、血浆中的雌二醇 Estradiol (E2) 。 简介: ResidueTest ® 雌二醇 ELISA 试剂盒用于定量检测牛奶和奶牛血清、血浆中雌二醇 (E2)的浓度。雌二醇 (1,3,5(10)-estratriene-3,17 β - 二醇 ; 17 β - 雌二醇 ; E21) 是 C18 类固醇激素
以更少量的样本完成对更多生物标志物的分析。由卓越的免疫试剂供应商开发,用于 Luminex® 平台,ProcartaPlex 使您可以在 25-50μL 的样本(血浆、血清、细胞培养上清液以及其他体液)。实现在 25-50μL 的样本中同时对多达 50 种因子进行检测与定量。该技术在多通道「类 ELISA」分析中,为每种靶标使用了经不同染色处理的捕获微球。相比于传统的三明治 ELISA 而言,ProcartaPlex 免疫分析可以在使用样本量少而时间相同的情况下,分析多达 50 倍
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells
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