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Swine Prothrombin ELISA Kit(猪凝

血酶原PT酶联免疫试剂盒)
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  • ¥4480
  • AssayMax
  • 0.3 ng/ml
  • 美国
  • EPP3022-1
  • 2026年04月17日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 供应商

      AssayPro

    • 检测范围

      0.3 ng/ml

    • 检测方法

      ELISA

    • 应用

      血清、血浆、细胞培养上清液

    • 样本

      50 μl

    • 规格

      96 wells

    AssayMax Swine Prothrombin ELISA Kit
    猪凝血酶原PT酶联免疫试剂盒

    Introduction
    Prothrombin is also known as Factor II. The conversion of Factor X to Xa changes prothrombin into its active form, thrombin, which then accelerates the formation of fibrin. The level of the plasma prothrombin in the circulating blood decreases during its passage through the pulmonary capillaries (1). The bleeding tendency in acute chloroform intoxication is due to deficiency in both plasma fibrinogen and plasma prothrombin (2). On the other hand, in severe Alzheimer's disease, prothrombin was localized within the wall and neuropil surrounding microvessels (3). It has been reported that plasma prothrombin level increases in sepsis patients (4), and in chronic gastrointestinal disorders (5).

    Principal of the Assay
    The AssayMax Swine Prothrombin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of swine prothrombin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine prothrombin in less than 4 hours. A monoclonal antibody specific for swine prothrombin has been pre-coated onto a 96-well microplate with removable strips. Prothrombin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for swine prothrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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    图标文献和实验
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    • ELISA (Enzyme-Linked ImmunoadSorbent Assay)

      and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells

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