AssayPro—Human alpha1-acid Gly

Introduction
Alpha-1-acid glycoprotein (AGP) or orosomucoid is an acute-phase plasma glycoprotein. It is synthesized in the liver and secreted into the plasma. The protein is a single polypeptide chain of 183 amino acids containing high carbohydrate content (45%) of its 41 kDa molecular weight (1). As a consequence of acute infections or inflammation, the plasma concentration of AGP increases considerably. The elevated serum level of AGP is associated with an increased risk of cardiovascular disease. Urinary AGP excretion rate predicts cardiovascular mortality in patients with Type II diabetes (2). AGP can be used as a marker for acute inflammation (3), chronic alcohol drinking (4), chronic kidney disease (5), and asthma (6).

Principal of the Assay
The AssayMax Human AGP ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive enzyme immunoassay technique that measures AGP in human plasma and serum in less than 3 hours. A polyclonal antibody specific for human AGP has been pre-coated onto a 96-well microplate with removable strips. AGP in standards and samples is competed by a biotinylated AGP sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.