Introduction Albumin, a serum hepatic protein, is the most abundant protein in serum. It contributes to the maintenance of oncotic pressure as well as the transport of hydrophobic molecules (1). Serum albumin level has been linked in clinical practice to several diseases. Low albumin levels can suggest liver disease (2), kidney disease (3), inflammation (4), shock (5), and malnutrition (6). On the other hand, high albumin levels usually reflect dehydration (7).
Principle of the Assay The AssayMax Mouse Albumin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of mouse albumin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures mouse albumin in less than 2 hours. A polyclonal antibody specific for mouse albumin has been pre-coated onto a 96-well microplate with removable strips. Albumin in standards and samples is competed with a biotinylated albumin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.