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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
AssayPro
- 检测范围:
0.15 ng/ml
- 检测方法:
ELISA
- 应用:
血清、血浆、细胞培养上清液
- 样本:
50 μl
- 规格:
96 wells
甲胎蛋白AFP酶联免疫试剂盒
Alpha-Fetoprotein (AFP) is a fetal-specific glycoprotein with a molecular weight of around 70 kDa. It is expressed in the embryonic liver, by cells of the vitelline sac and by the fetal intestinal tract in the first trimester of pregnancy (1). After birth, the synthesis of α-fetoprotein decreases rapidly. AFP level in adults is low but detectable (2). Alpha-fetoprotein has no known function in healthy adults. High level of α-fetoprotein in adult individual may be associated with a hepatocellular carcinoma (HCC), malignant tumor of the liver (1, 3). Thus, the concentration of α-fetoprotein in serum can be measured as a first step in HCC diagnosis (4 - 5). Moreover the elevated level of α-fetoprotein has been observed in lung cancer (6), gastric cancer (7 - 8), yolk sac tumor, and adenocarcinoma (9).
Principle of the Assay
The AssayMax α-Fetoprotein ELISA kit is designed for detection of human α-fetoprotein in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures α-fetoprotein in less than 4 hours. A polyclonal antibody specific for α-fetoprotein has been pre-coated onto a microplate. Human α-fetoprotein in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for α-fetoprotein, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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文献和实验. Compared with bayer Acs:180� Automated immunoassay system, the relative coefficient was 0.994(P 【Keywords】 alpha�fetoproteins; chemiluminescent measurements; quanti tative analysis 引言 甲胎蛋白(AFP)为最常用的肿瘤标志物之一. 是目前最好的可实际用于早期
作者:dwh1217 回复数:0000【求助】请推荐检测呼吸道病毒抗体试剂盒作者:星兰 回复数:0000【求助】上海哪个试验室有自动酶联斑点图像分析仪器?急作者:byday100 回复数:0000【请教】ELISA法测定大鼠肝组织匀浆TNF-alpha作者:yaocz96888 回复数:0000【讨论】北京做ELISA研究开发的朋友有没有想跳槽的作者:tedzhangbj 回复数:0000分析elisa结果的CurveExpert 1.38使用方法作者:cathryn167 回复
cytotoxic cell 抗体依赖细胞毒细胞 ADH antidiuretic hormone 抗利尿激素 AEC 3-amino-9-ethyl-carbazole 3-氨-9-乙基咔唑 AFP alpha(α)-fetal-protein 甲胎蛋白 AHA anti-histone antibody 抗组蛋白抗体 AKP alkaline phosphatase 碱性磷酸酶 ALP Alkaline Phosphatase 碱性
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