- ¥5530
- AssayPro
- 30 U/ml
- 美国
- CF1010
- 2026年09月23日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
AssayPro
- 检测范围：
30 U/ml
- 检测方法：
发色底物法
- 应用：
血清、血浆、细胞培养上清液
- 样本：
20 μl
- 规格：
96 wells
因子FX发色底物法活性试剂盒
Factor X (FX) is a plasma serine protease zymogen involved in the blood coagulation cascade. FX is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The FX heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the FX-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue factor/factor VIIa) pathway to give an active enzyme FXa. FXa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways (1 - 4).
Principle of Assay The AssaySense Human Factor X Chromogenic Activity Assay Kit is developed to determine human FX activity in plasma, serum, and cell culture. The assay measures the activation of zymogen FX to FXa by RVV. The amidolytic activity of the FXa is quantitated using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the FX enzymatic activity.
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文献和实验, these assays can readily be performed in a microplate with relatively few cells. Since a cytotoxic factor will reduce the rate of tetrazolium salt cleavage by a population of cells, these metabolic activity assays are frequently used to measure factor-induced
MINICHROMOSOME MICROTUBULE BINDING ASSAY
mM EGTA 1mM MgCl2 0.1mL 1M MgCl2 MINICHROMOSOME-MICROTUBULE BINDING ASSAY GROWTH OF CELLS. Grow 100mL of cells to OD600 =0.7-0.8 at 23o C. For good binding activity it has proved important to maintain cells in log phase
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
. Caspase enzymatic assay After incubation of the extracts in the presence or absence of activating stimuli (cytochrome c/dATP or active recombinant caspase), caspase enzymatic activity was measured
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