- ￥4130
- AssayMax
- 0.15 ng/ml
- 美国
- EA8201-1
- 2026年01月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
AssayPro
- 检测范围：
0.15 ng/ml
- 检测方法：
ELISA
- 应用：
血清、血浆、尿液、细胞培养上清液
- 样本：
50 μl
- 规格：
96 wells
人血清淀粉样蛋白SAP酶联免疫试剂盒
Introduction
Amyloid P component, serum (SAP) is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein (1). Serum amyloid P component is a normal plasma protein and a universal non-fibrillar constituent of amyloid deposits. Serum Amyloid P (SAP) is a pentraxin similar to C-reactive protein. SAP and CRP are suggested to associate with cardiovascular disease (2, 3). SAP is also suggested to link with Alzheimer's disease (4) and liver disease (5).
Principal of the Assay
The AssayMax Human Serum Amyloid P ELISA kit is designed for detection of human SAP in plasma, serum, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures SAP in less than 4 hours. A polyclonal antibody specific for SAP has been pre-coated onto a 96-well microplate with removable strips. SAP in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for SAP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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文献和实验to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
. The competitive effects of serum proteins on cell adhesion. J. Cell Sci. 71:17‐35. Engvall, E. and Perlmann, P. 1971. Enzyme‐linked immunosorbent assay (ELISA): Quantitative assay
in the circulation, and their levels are believed to reflect type II collagen synthesis. In this chapter we describe the development of a specific enzyme-linked immunosorbent assay (ELISA) for the measurement of the IIA form of PIINP (PIIANP) in serum based
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