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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
DAB Enhancing Solution
- 库存:
1
- 供应商:
甄准生物
- 规格:
30 ml
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文献和实验complex or the Vector ABC-peroxidase Elite (Catalog No. PK-6100) before finishing the previous step. Mix 5ml 1x PBS, two drops of Solution A, and two drops of Solution B, and allow to sit at 4°C for 30 min. prior to use. Apply enough to cover the sections
Bromodeoxyuridine Staining of Mammary Tissue
Triton X-100 100mL PBS 40 mL DAB Solution DAB Stock - 3'-3' diaminobenzadine, 2.5 mg/mL in 0.1M PO4, pH7.6. Unactivated DAB - 1 mL DAB + 19 mL 0.1M PO4 +450 mL 1% aq. NiCl2, pH7.6. Activated DAB - 10 mL unactivated DAB + 23 mL 30% H2O2.
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
solution (a small spoonful of nonfat dry milk in 40 mL PBS) Bovine serum albumin (BSA) 3,3''-diaminobenzidine tetrahydrochloride (DAB; Sigma D5637) Detergent (optional, see Step 2) Drosophila (see Steps 7-9) Entellan (EMD
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