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β-葡聚糖 及 β-葡聚糖检测试剂盒

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品牌: zzstandard
货号: 073-04811
供应商: 上海甄准生物科技有限公司
数量: 少量现货
规格: 10 mg
β-葡聚糖 073-04811  10 mg ( CAS NO: 9041-22-9) Grade: for Biochemistry
β-Glucan又名β-D-1,3-Glucan Beta-Glucan, Water Soluble
其他名称如β-Glucan ;beta-D-Glucan;Beta-1,3-glucan;b-Glucan;



检测试剂盒如下:

297-51501

SLP Reagent Set

微生物(细菌、真菌、酵母)检测试剂,可用多种测试方法

30-50T

295-53001

SLP Single Reagent Set

微生物(细菌、真菌、酵母)检测试剂

25T

293-58301

SLP-HS Single Reagent Set

微生物(细菌、真菌、酵母)检测试剂,高敏感型

20T


β-葡聚糖检测试剂盒
根据检测原理的不同,分为以下几种
297-51501 SLP Reagent Set(β-D-1,3-Glucan) 3mL (利用家蚕)

639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) (利用鲎)
Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen are removed from the lysate. The main reagent in this kit is the purified fraction added with the chromogenic synthetic substrate. This reagent is not reactive to endotoxin and specifically detects beta-glucan.

Principle of the test
The factor G in the purified fraction of the lysate reacts with (1->3)-beta-D-glucan. Then the factor G cascade reactions are activated. The clotting enzyme produced in the cascade reactions reacts with the substrate to release p-nitroaniline. The released p-nitroaniline is coupled with the diazocoupling reagent. Then the absorbance of the reaction mixture is measured. The amount of the p-nitroaniline is quantitatively determined.
BGSTAR can be used on a microplate reader by the endpoint method or the kinetic method. In the endpoint method, higher sensitivity can be obtained as the released p-nitroaniline is coupled with the diazo reagent and the absorptivity is enhanced. In the kinetic method, an operator can leave after starting the reaction and the measurable range is wider.

Ordering information
639-01051 BGSTAR A KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 vial x 3.0 mL, (3) Standard Curdlan, 1 vial x approx. 1,000 pg, (4) beta-Glucan-Free Water, 1 vial x 15 mL, (5) Diazocoupling Reagent, 1 vial x 4.8 mg, (6) Solvent for Diazocoupling Reagent 1, 1 vial x 12 mL, (7) Diazocoupling Reagent 2, 1 vial x 36 mg, (8) Diazocoupling Reagent 3, 1 vial x 8.4 mg

636-01061 BGSTAR B KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 x 3.0 mL

Note: With one vial of Main Reagent, 40 tests can be performed on a microplate reader. For the kinetic method, Diazocoupling Reagents are not used.
温馨提示:不可用于临床治疗。

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β-葡聚糖 及 β-葡聚糖检测试剂盒β-葡聚糖    073-04811       10 mg      ( CAS NO: 9041-22-9) Grade: for Biochemistryβ-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble其他名称如β-Glucan ;beta-D-Glucan;Beta-1,3-glucan;b-Glucan;β-葡聚糖 及 β-葡聚糖检测试剂盒检测试剂盒如下: 297-51501 SLP Reagent Set 微生物(细菌、真菌、酵母)检测试剂,可用多种测试方法 30-50T 295-53001 SLP Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂 25T 293-58301 SLP-HS Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂,高敏感型 20T β-葡聚糖检测试剂盒根据检测原理的不同,分为以下几种297-51501 SLP Reagent Set(β-D-1,3-Glucan)    3mL (利用家蚕)或者生物网,说明书见内。联系电话 639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) (利用鲎)Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen aziqibio073-04811
β-葡聚糖 073-04811  10 mg ( CAS NO: 9041-22-9) Grade: for Biochemistryβ-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble其他名称如β-Glucan ;beta-D-Glucan;Beta-1,3-glucan;b-Glucan;检测试剂盒如下: 297-51501 SLP Reagent Set 微生物(细菌、真菌、酵母)检测试剂,可用多种测试方法 30-50T 295-53001 SLP Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂 25T 293-58301 SLP-HS Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂,高敏感型 20T β-葡聚糖检测试剂盒根据检测原理的不同,分为以下几种297-51501 SLP Reagent Set(β-D-1,3-Glucan) 3mL (利用家蚕)639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) (利用鲎)Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen are removed from the lysate. The main reagent in this kit is the purifizzstandard073-04811
β-葡聚糖 073-04811 10 mg ( CAS NO: 9041-22-9) Grade: for Biochemistryβ-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble其他名称如β-Glucan ;beta-D-Glucan;Beta-1,3-glucan;b-Glucan;检测试剂盒如下: 297-51501 SLP Reagent Set 微生物(细菌、真菌、酵母)检测试剂,可用多种测试方法 30-50T 295-53001 SLP Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂 25T 293-58301 SLP-HS Single Reagent Set 微生物(细菌、真菌、酵母)检测试剂,高敏感型 20T β-葡聚糖检测试剂盒根据检测原理的不同,分为以下几种297-51501 SLP Reagent Set(β-D-1,3-Glucan) 3mL (利用家蚕)639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) (利用鲎)Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen are removed from the lysate. The main reagent in this kit is the purifiezzstandard297-51501
本试剂盒用于检测肽葡聚糖{peptidoglycan}和β-D-葡聚糖{(1→3)- β-D-glucan}。(西宝外贸部 译自日本Wako和光纯药 297-51501 SLP Reagent Set 3mL 试剂说明书)该试剂一般信息家蚕的血淋巴有一种自我保护机制,被称为“酚氧化酶前体级联系统”。当受外界入侵(如细菌和真菌)时,这个级联系统参与黑色素的形成并使它们免受袭击。这个系统由细菌中的肽葡聚糖和真菌、酵母中的β-葡聚糖引发,最后,该系统中的酚氧化酶被激活。也有人认为丝氨酸蛋白酶也参与了级联系统的反应,但是没有从这方面进行阐述。已消毒的SLP包括了所有参与级联系统的要素。在这个系统中,复原溶液中的DOPA犹如酚氧化酶底物,这是由肽葡聚糖或β-葡聚糖引发的。DOPA由酚氧化酶氧化,在反应混合物中形成黑色素。肽葡聚糖是细菌壁的组成成分,而β-葡聚糖广泛的分布于真菌的细胞壁中。因此,SLP通过测量黑色素的形成情况,进而用于定量和检测细菌和真菌的污染。特点1、 用于肽葡聚糖和β-葡聚糖的定量,检测敏感2、 使用酶标测定仪,能对肽葡聚糖和β-葡聚糖准确定量3、 不需任何特殊器械即可通过肉眼检测出细菌和真菌的污染4、 冻干SLP的稳定性在2-10℃至少可保存18个月。原理 297-51501 SLP Reagent Set 3mL Wako和光纯药SLP的作用机制见图1所示。不是肽葡聚糖就是β-葡聚糖会与识别蛋白相偶联,而被称为PGRP或βGRP。酚氧化酶前体转化为酚氧化酶。然后,酚氧化酶催化DOPA氧化,同时,混合液中产生黑色素。过一段时间,任何人可通过肉眼判断反应混合物的颜色变化。当使用酶标测定仪时,通过测定反应的初动时间和由颜色变化而导致辞的吸光度的变化,即可对肽葡聚糖或β-葡聚糖进行定量。所需试剂所有试剂均存放于2-10℃.1、 冻干SLP 每小瓶3ml2、 底物,冻干 每小瓶3ml3、 稀释液 每小瓶4ml提前准备试剂:A. 加3ml稀释液入底物中,轻轻搅匀至底物完全溶解,避免与瓶口接触。B. 所有溶液加入冻干的SLP小瓶中,轻轻搅匀至SLP完全溶解,同样,避免与瓶口接触。此溶液即为SLP检测液。操作步骤1、 眼检测使用酶标板A. 对倍稀释参考物--肽葡聚糖或β-葡聚糖,每孔加入0.05ml的稀释液和样zzstandard297-51501

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