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- 库存：
少量现货
- 供应商：
上海甄准生物科技有限公司
- 规格：
10 mg
β-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble
其他名称如β-Glucan ；beta-D-Glucan；Beta-1,3-glucan；b-Glucan；
检测试剂盒如下：
| 297-51501 |
SLP Reagent Set |
微生物（细菌、真菌、酵母）检测试剂，可用多种测试方法 |
30-50T |
| 295-53001 |
SLP Single Reagent Set |
微生物（细菌、真菌、酵母）检测试剂 |
25T |
| 293-58301 |
SLP-HS Single Reagent Set |
微生物（细菌、真菌、酵母）检测试剂,高敏感型 |
20T |
β-葡聚糖检测试剂盒
根据检测原理的不同，分为以下几种
297-51501 SLP Reagent Set（β-D-1,3-Glucan） 3mL （利用家蚕）
639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) （利用鲎）
Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen are removed from the lysate. The main reagent in this kit is the purified fraction added with the chromogenic synthetic substrate. This reagent is not reactive to endotoxin and specifically detects beta-glucan.
Principle of the test
The factor G in the purified fraction of the lysate reacts with (1->3)-beta-D-glucan. Then the factor G cascade reactions are activated. The clotting enzyme produced in the cascade reactions reacts with the substrate to release p-nitroaniline. The released p-nitroaniline is coupled with the diazocoupling reagent. Then the absorbance of the reaction mixture is measured. The amount of the p-nitroaniline is quantitatively determined.
BGSTAR can be used on a microplate reader by the endpoint method or the kinetic method. In the endpoint method, higher sensitivity can be obtained as the released p-nitroaniline is coupled with the diazo reagent and the absorptivity is enhanced. In the kinetic method, an operator can leave after starting the reaction and the measurable range is wider.
Ordering information
639-01051 BGSTAR A KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 vial x 3.0 mL, (3) Standard Curdlan, 1 vial x approx. 1,000 pg, (4) beta-Glucan-Free Water, 1 vial x 15 mL, (5) Diazocoupling Reagent, 1 vial x 4.8 mg, (6) Solvent for Diazocoupling Reagent 1, 1 vial x 12 mL, (7) Diazocoupling Reagent 2, 1 vial x 36 mg, (8) Diazocoupling Reagent 3, 1 vial x 8.4 mg
636-01061 BGSTAR B KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 x 3.0 mL
Note: With one vial of Main Reagent, 40 tests can be performed on a microplate reader. For the kinetic method, Diazocoupling Reagents are not used.
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文献和实验，由糊精合成葡聚糖。葡聚糖的种类很多，仅由 D-葡萄糖组成，主链是α（ 1， 6）键，也有α（ 1， 4）或α（ 1， 3）键的支链。葡萄糖为白色粉末，在水中加一点点即可产生很强的右旋性。医药上用作代用血浆。 [2]是以葡萄糖为组成糖的多糖的总称。由于 D-葡萄糖残基彼此间结合样式的不同而分为多种，广泛分布于微生物、植物、动物界。其中代表性的有细菌的多缩葡萄糖（由α -1， 6键的主链上支出以α -1， 4和α -1， 6键的侧链），褐藻类的海带多糖（ lami-narin）（主要以β
愈创葡聚糖 callose 系曼金（ L． Mangin． 1892）在筛管的愈伤组织板用显微化学方法所发现的物质。系靠β -1， 3键形成的葡萄糖聚合物，卷成螺旋状结合一起的。
催化核苷二磷酸葡萄糖的糖苷基转移而生成葡聚糖的酶。淀粉合成酶（2-1， 4-葡聚糖合成酶， EC2． 4． 1． 21）也是一种葡聚糖合成酶，但本词条所说的葡聚糖合成酶一般指与细胞壁合成有关的β -1， 4-葡聚糖合成酶和β -1， 3-葡聚糖合成酶而言。在醋酸菌中含有催化由尿苷二磷酸葡萄糖（ UDPG）合成纤维素的酶。在高等植物中也有与膜结合的具有同样活性的酶，但对基质的特异性还不清楚。以 UDPG为基质时产物不仅有β -1， 4-葡聚糖也混有β -1， 3-葡聚糖，基质浓度增高则β
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