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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Yeast Protein Extract: 酵母蛋白质提取
- 库存：
1
- 供应商：
甄准生物
- 规格：
3 vials x 0.2 mL
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文献和实验Yeast Extract for Friis Medium
1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.2.Place in incubator at 37°C for 20 minutes.3.Heat to 90-100°C for 5 minutes.4.Cool on ice.5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C).6.Filter supernatant
Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J.Leatherwood,Ptashne lab) last modified Mon,Aug 27,2001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is added
In vitro transcription with yeast nuclear extract
In vitro transcription with yeast nuclear extract Steve Hahn Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before use to eliminate any stray RNAses
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