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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 英文名：
Yeast Total Lipid Extract (S. cerevisiae)
- 库存：
999+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
N/A
别名：Yeast Total Lipid Extract (S. cerevisiae)
吸湿性：Yes
稳定性：3 Months
存储：冷冻-20°C
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文献和实验Yeast Nuclear Extract (Small Scale)
,7.5 250 ml 1.5g Tris in 250 ml H2O pH to 7.5 with HCl Before use,add 4.6 mg DTT/ml YPD/S 2.5 liters at room temp.(YPD with 1M sorbitol) 25g Yeast extract 50g Bactopeptone 50g Dextrose (glucose) 455g Sorbitol H2O to 2.5 liters total YPD/S 1
and maximize lipid yield. Once lipids have been extracted, fatty acids are removed and the resulting water-soluble glycerophosphoinositol phosphates are analysed by anion-exchange HPLC. This chapter describes how to extract and quantify the inositol lipids of S
Purification of YAC-Containing Total Yeast DNA
There are many methods describing the preparation of DNA from Saccharomyces cerevisiae . The method described in this chapter is a modification of those described by Olson et al. (1 ) and Philippsen et al. (2 ). Although simpler techniques
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