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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
SOLVENT 142 HT
- 库存：
1
- 供应商：
甄准生物
- 保存条件：
RT
- 规格：
1L
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文献和实验Real-Time qRT-PCR standard curves... efficiency is too h
I may get excellent melting curves for each dilution, but the slope for my standard curve may fall at around -2.6 (~142% efficiency)! Can anyone offer some explanations for this strange dilemma? Consequently, can anyone offer some suggestions to solve this problem
, 50 µg streptomycin and 50 µM 2-ME in the absence or presence of HAT or HT for selection (I-20). Procedure: NOTE: All washes are at 1,000 rpm for 10 minutes at 4° C using serum free media Tissue Collection: 1. Sacrifice animal
glutamine, 1 mM sodium pyruvate, 50 U penicillin, 50 µg streptomycin and 50 µM 2-ME in the absence or presence of HAT or HT for selection (I-20). Procedure: NOTE: All washes are at 1,000 rpm for 10 minutes at 4° C using serum free media
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