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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
2-Oleoyl Glycerol (80%)
- 库存:
1
- 供应商:
甄准生物
- CAS号:
3443-84-3
- 保存条件:
-86˚C Freezer, Under Inert Atmosphere
- 规格:
2.5mg / 25mg
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文献和实验Long Term Storage of Bacterial Strains
Purpose: Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to prepare a frozen stock of newly acquired or created strains for the archives as soon
; store 5X BRB80 stock at 4¡C) 10X IB : 500 mM K-glutamate; 5 mM MgCl2 , (pH of 1X ~ 7.0; store 10X stock at -20¡C; see labeling section for details on making up IB) Cushion : 60% glycerol in 1X BRB80 (warm = 37¡C) 100 mM GTP Glycerol
Thaw tubulin Adjust solution to a final buffer concentration equivalent of glycerol PB Incubate at 37 o C for 30 min. Layer tubulin onto cushions of BRB80 containing 60% (v/v) glycerol
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