Ethidium-d1 Bromide

1) Cat. No.: TRC-E890703; 2) Chemical Name: Ethidium-d1 Bromide; 3) CAS No.: ; 4) Mol. Formula: C21H19DBrN3 ; 5) Mol. Weight: 395.31; 6) Packing Size: 2.5mg / 25mg; 7) Appearence: ; 8) Application Notes: Labelled Ethidium bromide (EtBr) (E890700). It is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma). ; 9) Synonym: 3,8-Diamino-5-(ethyl-d1)-6-phenylphenanthridinium Bromide; 2,7-Diamino-10- (ethyl-d1)-9-phenylphenanthridinium Bromide; 2,7-Diamino-9-phenyl-10-(ethyl-d1) phenanthridinium Bromide; 2,7-Diamino-9-phenylphenanthridine-d1 Ethobromide; Dromilac-d1; Homidium-d1 Bromide; ; 10) Solubility: ; 11) Storage condition: ; 12) References: Waring, M.J., et al.: J. Mol. Biol., 13, 269 (1965), Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),