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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Cyanine 3 Maleimide, Potassium Salt (~90%)
- 库存:
1
- 供应商:
甄准生物
- 保存条件:
Refrigerator, Under Inert Atmosphere
- 规格:
0.5mg / 5mg
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文献和实验Plasmid DNA Isolation, Alkaline Lysis(碱裂解法分离质粒DNA)
volume to 500ml with water. Soln 3 (B): per 100 mls:29.4 g potassium acetate/5 ml 90% formic acid/add water to bring to final volume. Filter if turbid. Protocol: Culture Volume 30-35 ml 1.5 ml 500 ml 1. Centrifuge to pellet and add soln 1 on ice for 15-30 min
Site-directed hydroxy radical mapping of nucleosome positions in vitro
for site directed mapping is BABE (bromoacetamidobenzyl EDTA (Meares et al ., 1984)). However, the predominant side reaction of common thiol-active functional groups such as bromoacetamide and maleimide is with lysine. There are a very large number
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
(Rentrop et al., 1986) or gelatin-coated slides; air-dry O/N. Sections up to 90 m m thick (the thickest we have tried) have been successfully stained. Gelatin Solution for subbing slides 2 g gelatin 0.1 g chromium potassium sulfate (chrome
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