小鼠肠平滑肌细胞生长特性：贴壁；培养条件：37℃、5%CO₂

小鼠肠平滑肌细胞
英文名称：Mouse Intestinal Smooth Muscle Cells
规格：1×10^6 cells/管（冻存管，1mL/管）；或1×10^6 cells/T25培养瓶（复苏后培养）
产品简介：小鼠肠平滑肌细胞属于小鼠源肠来源的正常细胞。该细胞在体外可稳定增殖，呈贴壁生长，形态为上皮样、多角形，广泛用于细胞生物学、毒理学评价及体外模型构建等研究方向。本品经种属与形态学鉴定，种属来源明确、形态特征典型，可提供稳定可重复的实验结果。
基本信息：细胞名称：小鼠肠平滑肌细胞；种属：小鼠；组织来源：肠；细胞类型：平滑肌细胞；生长特性：贴壁；完全培养基：RPMI-1640 + 10% FBS；培养条件：37℃、5% CO2；传代比例：1:2-1:3；冻存条件：90% FBS + 10% DMSO；生物安全等级：BSL-2
产品优势：①种属鉴定合格，种属来源清楚，形态典型；②代次低、活力高，复苏后存活率≥90%，贴壁/增殖状态稳定；③支原体、细菌、真菌检测均为阴性，无外源因子污染；④批次稳定，可长期重复供货，配套完整培养方案；⑤提供鉴定报告与批次质检记录，数据可追溯
产品售后：收到细胞后7天内，如出现污染、贴壁不良、存活率低或鉴定不符等问题，经核实可免费更换或补发；提供全程技术支持（培养基配制、复苏传代、冻存与实验设计建议）；可应要求出具STR/种属鉴定报告、支原体检测报告及批次质检记录。
储存条件：液氮（-196℃）气相长期保存；-80℃冰箱仅可短期存放（不超过3个月）；避免反复冻融，冻存管取出后于37-40℃水浴快速解冻并在2分钟内转移至完全培养基。
运输条件：冻存管采用干冰运输（全程维持-70℃以下）；T25培养瓶采用常温运输（灌满完全培养基、密封防漏），收到后用75%酒精消毒表面，静置培养箱24小时后再观察处理。
物种来源：种属：小鼠（Mouse）；组织来源：肠
细胞形态：上皮样、多角形，贴壁生长，呈单层紧密排列
生长状态：贴壁生长，细胞贴附于培养瓶底面单层增殖，汇合度达80%-90%时传代
年限：液氮条件下长期保存，有效保存期5年；复苏后建议连续传代不超过10代（原代细胞建议3-5代内使用），代次过高可能引起表型漂移与增殖能力下降。
免疫类型：小鼠源细胞，属同种移植背景，可在免疫功能健全的同系宿主中移植（如C57BL/6或BALB/c，需与细胞品系一致）；体外培养无需考虑免疫排斥。
质量控制与检测：种属鉴定无误、形态学特征典型；支原体检测阴性；细菌、真菌无菌检测阴性；细胞活力≥90%；内毒素检测合格
传代方法：弃去旧培养基，PBS（无钙镁）润洗1次，加入0.25%胰蛋白酶-EDTA消化液覆盖细胞层，37℃消化1-3分钟，镜下见细胞回缩变圆、间隙增大即加入含血清完全培养基终止消化，1000rpm离心5分钟，弃上清后用完全培养基重悬，按1:2-1:3比例接种
培养条件：37℃、5% CO2、饱和湿度培养箱培养；每2-3天换液一次，细胞汇合度80%-90%时按1:2-1:3传代
培养基：RPMI-1640 + 10% FBS
Mouse Intestinal Smooth Muscle Cells
English Name: Mouse Intestinal Smooth Muscle Cells
Specifications: 1×10^6 cells/vial (cryovial, 1 mL/vial); or 1×10^6 cells/T25 flask (cultured after recovery)
Mouse Intestinal Smooth Muscle Cells is a normal cell line derived from mouse intestine. These cells proliferate stably in vitro, showing adherent growth, an epithelioid and polygonal morphology, and are widely used in research on cell biology, toxicological evaluation, and in vitro model construction. This product has passed species and morphological identification, with a clear species origin and typical morphological features, ensuring stable and reproducible experimental results.
Basic Information: Cell name: Mouse Intestinal Smooth Muscle Cells; Species: Mouse; Tissue source: Intestine; Cell type: Smooth muscle cells; Growth characteristics: Adherent; Complete medium: RPMI-1640 + 10% FBS; Culture conditions: 37°C, 5% CO2; Subculture ratio: 1:2–1:3; Cryopreservation: 90% FBS + 10% DMSO; Biosafety level: BSL-2
Product Advantages: ① Species identification passed, with a clear species origin and typical morphology; ② Low passage number and high viability, with a post-thaw survival rate of ≥90% and stable adherence/proliferation; ③ Negative for mycoplasma, bacteria, and fungi, with no adventitious agent contamination; ④ Stable batches for long-term repeat supply, with a complete culture protocol; ⑤ Identification reports and batch quality inspection records available for traceability
After-Sales Service: Within 7 days of receiving the cells, if issues such as contamination, poor adherence, low viability, or failed authentication occur, free replacement or resupply will be provided after verification. Full technical support is available (medium preparation, recovery and subculture, cryopreservation, and experimental design advice), and STR/species identification reports, mycoplasma testing reports, and batch quality inspection records can be issued upon request.
Storage Conditions: Long-term storage in the gas phase of liquid nitrogen (-196°C); the -80°C freezer is suitable only for short-term storage (no more than 3 months). Avoid repeated freeze-thaw cycles; after removing the cryovial, thaw it rapidly in a 37-40°C water bath and transfer it to complete medium within 2 minutes.
Shipping Conditions: Cryovials are shipped on dry ice (maintained below -70°C throughout); T25 flasks are shipped at ambient temperature (filled with complete medium and sealed against leakage). Upon receipt, disinfect the surface with 75% ethanol and leave the cells in the incubator for 24 hours before observation and handling.
Species and Source: Species: Mouse; Tissue source: Intestine
Cell Morphology: Epithelioid and polygonal; adherent growth with a tightly packed monolayer arrangement
Growth Status: Adherent growth; cells attach to the culture flask surface and proliferate as a monolayer; subculture when confluence reaches 80%-90%
Storage Life and Passage Limit: Long-term preservation under liquid nitrogen with a valid storage period of 5 years; after recovery, continuous passaging of no more than 10 passages is recommended (primary cells are recommended for use within 3-5 passages), as an excessively high passage number may cause phenotypic drift and reduced proliferative capacity.
Immunological Background: Mouse cells, with a syngeneic transplantation background; they can be transplanted into immunocompetent syngeneic hosts (e.g., C57BL/6 or BALB/c, consistent with the cell strain), while in vitro culture does not require consideration of immune rejection.
Quality Control and Testing: Species identification confirmed, with typical morphological features; mycoplasma testing negative; sterility testing negative for bacteria and fungi; cell viability ≥90%; endotoxin testing passed
Subculture Method: Discard the old medium, rinse once with PBS (without calcium and magnesium), add 0.25% trypsin-EDTA solution to cover the cell layer, and digest at 37°C for 1-3 minutes; once the cells round up and separate under the microscope, add serum-containing complete medium to stop digestion, centrifuge at 1000 rpm for 5 minutes, discard the supernatant, resuspend in complete medium, and inoculate at a ratio of 1:2-1:3
Culture Conditions: Culture in a 37°C, 5% CO2, saturated-humidity incubator; renew the medium every 2-3 days and subculture at a ratio of 1:2-1:3 when confluence reaches 80%-90%
Culture Medium: RPMI-1640 + 10% FBS

FAQ（选取前2个问题）	问：多久可以传代	答：一般情况下70%–80%可以传代
	问：如何发货	答：复苏好的T25瓶发货 ，冻存的干冰发货