143B/GFP/Luc细胞，143B/GFP/Luc绿色荧

143B/GFP/Luc绿色荧光+荧光素酶双标记人骨肉瘤细胞株(STR鉴定正确)
英文名称：143B/GFP Luciferase-Expressing GFP-Labeled Human Osteosarcoma Cells
规格：1×10^6 cells/管（冻存管，1mL/管）；或1×10^6 cells/T25培养瓶（复苏后培养）
产品简介：143B/GFP/Luc绿色荧光+荧光素酶双标记人骨肉瘤细胞株属于人源肿瘤细胞系/细胞株，稳定表达荧光素酶（Luc），稳定表达绿色荧光蛋白（GFP）。该细胞在体外可稳定增殖，呈贴壁生长，形态为多角形或梭形，广泛用于肿瘤发生发展机制、药物敏感性评价、基因功能研究及体内外移植模型构建等研究方向。本品经STR图谱鉴定，与标准STR图谱一致，无交叉污染，可提供稳定可重复的实验结果。
基本信息：细胞名称：143B/GFP/Luc绿色荧光+荧光素酶双标记人骨肉瘤细胞株；种属：人；组织来源：骨肉瘤；细胞类型：骨肉瘤；生长特性：贴壁；完全培养基：DMEM高糖 + 10% FBS；培养条件：37℃、5% CO2；传代比例：1:2-1:3；冻存条件：90% FBS + 10% DMSO；生物安全等级：BSL-2
产品优势：①STR图谱鉴定合格，与权威STR数据库一致，无HeLa等交叉污染；②代次低、活力高，复苏后存活率≥90%，贴壁/增殖状态稳定；③支原体、细菌、真菌检测均为阴性，无外源因子污染；④批次稳定，可长期重复供货，配套完整培养方案；⑤荧光/抗原标记背景清晰，可直接用于示踪、成像与免疫学评价
产品售后：收到细胞后7天内，如出现污染、贴壁不良、存活率低或鉴定不符等问题，经核实可免费更换或补发；提供全程技术支持（培养基配制、复苏传代、冻存与实验设计建议）；可应要求出具STR/种属鉴定报告、支原体检测报告及批次质检记录。
储存条件：液氮（-196℃）气相长期保存；-80℃冰箱仅可短期存放（不超过3个月）；避免反复冻融，冻存管取出后于37-40℃水浴快速解冻并在2分钟内转移至完全培养基。
运输条件：冻存管采用干冰运输（全程维持-70℃以下）；T25培养瓶采用常温运输（灌满完全培养基、密封防漏），收到后用75%酒精消毒表面，静置培养箱24小时后再观察处理。
物种来源：种属：人（Human）；组织来源：骨肉瘤；经STR鉴定确认种属与组织来源
细胞形态：多角形或梭形，贴壁生长，形态均一
生长状态：贴壁生长，细胞贴附于培养瓶底面单层增殖，汇合度达80%-90%时传代
年限：液氮条件下长期保存，有效保存期5年；复苏后建议连续传代不超过10代（原代细胞建议3-5代内使用），代次过高可能引起表型漂移与增殖能力下降。
免疫类型：人源细胞，属异种移植背景；进行体内移植时需使用免疫缺陷小鼠（如BALB/c nude、NOD-SCID、NSG），体外培养不涉及免疫排斥；如用于免疫学实验应关注人源MHC与小鼠免疫系统的匹配性。
质量控制与检测：STR图谱鉴定与标准一致（≥20个位点）；支原体检测阴性；细菌、真菌无菌检测阴性；细胞活力≥90%；内毒素检测合格
传代方法：弃去旧培养基，PBS（无钙镁）润洗1次，加入0.25%胰蛋白酶-EDTA消化液覆盖细胞层，37℃消化1-3分钟，镜下见细胞回缩变圆、间隙增大即加入含血清完全培养基终止消化，1000rpm离心5分钟，弃上清后用完全培养基重悬，按1:2-1:3比例接种
培养条件：37℃、5% CO2、饱和湿度培养箱培养；每2-3天换液一次，细胞汇合度80%-90%时按1:2-1:3传代
培养基：DMEM高糖 + 10% FBS
143B/GFP Luciferase-Expressing GFP-Labeled Human Osteosarcoma Cells (STR authentication verified)
English Name: 143B/GFP Luciferase-Expressing GFP-Labeled Human Osteosarcoma Cells
Specifications: 1×10^6 cells/vial (cryovial, 1 mL/vial); or 1×10^6 cells/T25 flask (cultured after recovery)
143B/GFP Luciferase-Expressing GFP-Labeled Human Osteosarcoma Cells is a tumor cell line of human origin, stably expressing luciferase (Luc), stably expressing green fluorescent protein (GFP). These cells proliferate stably in vitro, showing adherent growth, a polygonal or spindle-shaped morphology, and are widely used in research on tumor initiation and progression mechanisms, drug sensitivity evaluation, gene function studies, and the construction of in vivo and in vitro transplantation models. This product has passed STR profiling and matches the reference STR profile, with no cross-contamination, ensuring stable and reproducible experimental results.
Basic Information: Cell name: 143B/GFP Luciferase-Expressing GFP-Labeled Human Osteosarcoma Cells; Species: Human; Tissue source: Osteosarcoma; Cell type: Osteosarcoma; Growth characteristics: Adherent; Complete medium: High-glucose DMEM + 10% FBS; Culture conditions: 37°C, 5% CO2; Subculture ratio: 1:2–1:3; Cryopreservation: 90% FBS + 10% DMSO; Biosafety level: BSL-2
Product Advantages: ① STR profiling passed and consistent with the authoritative STR database, with no HeLa or other cross-contamination; ② Low passage number and high viability, with a post-thaw survival rate of ≥90% and stable adherence/proliferation; ③ Negative for mycoplasma, bacteria, and fungi, with no adventitious agent contamination; ④ Stable batches for long-term repeat supply, with a complete culture protocol; ⑤ A clean fluorescent/antigen marker background, suitable directly for tracing, imaging, and immunological evaluation
After-Sales Service: Within 7 days of receiving the cells, if issues such as contamination, poor adherence, low viability, or failed authentication occur, free replacement or resupply will be provided after verification. Full technical support is available (medium preparation, recovery and subculture, cryopreservation, and experimental design advice), and STR/species identification reports, mycoplasma testing reports, and batch quality inspection records can be issued upon request.
Storage Conditions: Long-term storage in the gas phase of liquid nitrogen (-196°C); the -80°C freezer is suitable only for short-term storage (no more than 3 months). Avoid repeated freeze-thaw cycles; after removing the cryovial, thaw it rapidly in a 37-40°C water bath and transfer it to complete medium within 2 minutes.
Shipping Conditions: Cryovials are shipped on dry ice (maintained below -70°C throughout); T25 flasks are shipped at ambient temperature (filled with complete medium and sealed against leakage). Upon receipt, disinfect the surface with 75% ethanol and leave the cells in the incubator for 24 hours before observation and handling.
Species and Source: Species: Human; Tissue source: Osteosarcoma; Species and tissue origin confirmed by STR profiling
Cell Morphology: Polygonal or spindle-shaped; adherent growth with a uniform morphology
Growth Status: Adherent growth; cells attach to the culture flask surface and proliferate as a monolayer; subculture when confluence reaches 80%-90%
Storage Life and Passage Limit: Long-term preservation under liquid nitrogen with a valid storage period of 5 years; after recovery, continuous passaging of no more than 10 passages is recommended (primary cells are recommended for use within 3-5 passages), as an excessively high passage number may cause phenotypic drift and reduced proliferative capacity.
Immunological Background: Human cells, with a xenotransplantation background; in vivo transplantation requires immunodeficient mice (e.g., BALB/c nude, NOD-SCID, NSG), while in vitro culture does not involve immune rejection; for immunological experiments, the compatibility between human MHC and the murine immune system should be considered.
Quality Control and Testing: STR profiling consistent with the reference standard (≥20 loci); mycoplasma testing negative; sterility testing negative for bacteria and fungi; cell viability ≥90%; endotoxin testing passed
Subculture Method: Discard the old medium, rinse once with PBS (without calcium and magnesium), add 0.25% trypsin-EDTA solution to cover the cell layer, and digest at 37°C for 1-3 minutes; once the cells round up and separate under the microscope, add serum-containing complete medium to stop digestion, centrifuge at 1000 rpm for 5 minutes, discard the supernatant, resuspend in complete medium, and inoculate at a ratio of 1:2-1:3
Culture Conditions: Culture in a 37°C, 5% CO2, saturated-humidity incubator; renew the medium every 2-3 days and subculture at a ratio of 1:2-1:3 when confluence reaches 80%-90%
Culture Medium: High-glucose DMEM + 10% FBS

FAQ（选取前2个问题）	问：多久可以传代	答：一般情况下70%–80%可以传代
	问：如何发货	答：复苏好的T25瓶发货 ，冻存的干冰发货