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SSQ092 "水土中总磷酸盐检测试剂盒(分光光度法50T/48S)
Water / Soil Sample Total Phosphate Content Assay Kit (Spectrophotometry) " 50T/48S 盒 530.00
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文献和实验Sample Preparation for Microinjection
protocol is suitable for purifying RNA solutions [6]. As for DNA, RNA is dissolved in bidist. water after purification. Concentrations of 1–2 µg/ml should be used for mRNA and up to 10 µg/ml for total RNA. Storage Store at –80°C in small aliquots
Cloning of small RNAs with 5’ phosphate and 3’ OH ends
temperature 75% EtOH. Allow the RNA pellet to air dry then dissolve the RNA in total of 5.7µl of DEPC-treated water (we used Ambion’s nuclease-free water). 5’ Adaptor Ligation and Purification Heat the RNA for 30 seconds at 90°C
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sample. Add 0.8 ml Buffer SLX Mlus. Vortex at maximum speed for 3-5 minute to lyse samples. For the best result, A Mixer Mill, such as Fastprep-24, Mixer Mill MM 300
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