LD Biopharma Recombinant Human

Recombinant Human ZNF746 Protein，品牌LD Biopharma，货号HTF-0270，规格50 µg / Vial。 基因符号：ZNF746 (PARIS) 登录号：NP_689770 物种：Human 质量控制：Purity: > 90% by SDS-PAGE. 制剂组成：0.5 mg / ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT, Glycerol and others. 英文说明：Human ZNF746 (PARIS) is a 644 amino acid protein that contains a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues. Recent data indicated that ZNF746 accumulated in models of parkin inactivation and in human Parkinson’s disease (PD). ZNF746 represses the expression of the transcriptional co-activator, PGC-1a and the PGC-1a target gene, NRF-1 by binding to insulin response sequences in the PGC-1a promoter. Meanwhile, ZNF746 is also regulated by E3 ubiquitin ligase (parkin) and play its role in the progress of PD disease. Full-length human ZNF746 cDNA (643aa, derived from BC068505) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. 应用信息：May be used for in vitro ZFP746 mediated gene transcription regulation study in neuronal cells by intracellular delivery of this protein with ZFP746 protein delivery reagent such as ProFectin reagent kit. May be used for ZFP746 mapping protein-protein interaction. May be used as ZFP746 specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays, such as E3 ubiquitin ligase assay (parkin). Potential biomarker protein / therapeutic target protein for cancer prognosis and cancer treatment drug development. As native human ZFP746 antigen for specific antibody production. 本品仅供科研使用。实际构建、质量控制、保存及使用要求请以批次文件为准；网站目录不代表当前库存或实际供货承诺。