LD Biopharma Recombinant Human

Recombinant Human Fibronectin FN3 Domain-8 Protein，品牌LD Biopharma，货号HRP-1278，规格100 µg/ Vial。 基因符号：FN-III domain 8 登录号：NP_997647 物种：Human 质量控制：Purity: > 90% by SDS-PAGE. 制剂组成：1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. 英文说明：Extracellular matrix protein fibronectin (FN) plays an important role in cell adhesion. FN contains FN type I, II and III domains. The FN III repeat is generally about 90 amino acid long and is composed of seven b-strands, forming two antiparallel b-sheets. In the C-terminal of human fibronectin region, there are 16 FN-III repeat domains. FN III domain derived proteins have been demonstrated to bind various proteins with high affinity. To decode the biological function of each FN-III domain, all 16 FN-III domains were separately cloned and expressed in E.coli as soluble functional proteins. So coating them as matrix proteins in culture dish may provide a unique functional assay for regulating human ES cell lineage specific differentiation. Full-length third FN-III domain of Human fibronectin cDNA was constructed by fully synthetic gene synthesis with codon optimization and expressed with N-terminal 206 aa of human Alpha fetal protein (AFP) fusion protein. This protein was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. 应用信息：1. May be used for in vitro human ES lineage specific differentiation regulations study coating with this protein. 2. May be used for in vitro protein-protein interaction mapping. 3. As antigen for specific antibody production. 本品仅供科研使用。实际构建、质量控制、保存及使用要求请以批次文件为准；网站目录不代表当前库存或实际供货承诺。