- ￥1975 - 26255
- 翌圣生物
- 96440ES
- 2026年10月15日
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- 文献和实验
- 技术资料
- 英文名：
Recombinant SARS-associated coronavirus 2 3CLpro/3C-like Protease Protein (E166V)
- 保存条件：
-85~-65℃保存，收到货之日起，有效期1年。建议分装蛋白为一次实验使用量，分装后，-80℃冻存。避免反复冻融。
- 规格：
25 μg(96440ES25)/100 μg(96440ES60)/500 μg(96440ES76)
| 规格： | 25 μg(96440ES25) | 产品价格： | ￥1975 |
|---|---|---|---|
| 规格： | 100 μg(96440ES60) | 产品价格： | ￥7885 |
| 规格： | 500 μg(96440ES76) | 产品价格： | ￥26255 |
产品描述：
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins . The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.产品属性：
| 产品别称 | 3CL Protease;3CL-Mpro;M Proteinase;Mpro |
| 种属 | Virus |
| 表达序列 | Ser1-Gln306(E166V) |
| Accession | YP_009725301.1 |
| 标签 | No Tag |
| 表达系统 | E.coli |
| 分子量 | The protein has a predicted MW of 33.80 kDa same as Bis-Tris PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE |
| 内毒素 | Less than 1 EU per μg by the LAL method. |
| 制剂 | Supplied as 0.22μm filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5). |
| 外观 | Liquid |
产品特色：
高活性、高纯度、低内毒素、高批间一致性储存条件：
-85~-65℃保存，收到货之日起，有效期1年。建议分装蛋白为一次实验使用量，分装后，-80℃冻存。避免反复冻融。风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural
has been purified and characterized (3 ,6 –8 ). The enzyme is a 204-residue monomer of 24,838 Dalton with a pI of 10.59 and optimal activity at 45�C and pH 8.0. The recombinant enzyme is stimulated by an 11-amino acid cleavage fragment from viral protein pre-VI
Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
with a protein kinase regulating its activity in an adenosine triphosphate-dependent manner. When native or recombinant PDE2 is immunoprecipitated from PC12 cells using an antibody to the amino terminus in a buffer containing Lubrol 12A9, protease inhibitors
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